TECHNOLOGICAL AND ANALYTICAL STUDY OF WATER EXTRACTS FROM THE RHIZOMES OF THE HOOD

Aim. Study of the technology of making water extracts from the rhizomes of badan with the establishment of their quality indicators. Materials and Methods. The object of the study was water extracts made from powdered balanic rhizomes in filter bags (1.5 g) and from crushed rhizomes of baked beans packed in packs (50 g). From this raw material, aqueous extracts were prepared by the method of hot infusion with water according to instructions on the packaging with raw materials. Identification in extracts of arbutin was carried out by color reaction with iron oxide ferrous sulfate, thinlayer chromatography of extracts on Sorbfill plates in the solvent system ethyl acetate-formic acid-water. Developers: 1% alcohol solution of 2,6-dichloroquinone chloroimide, 2% sodium carbonate solution. Tannins were identified with a 1% solution of iron ammonium alum. The quantitative determination of arbutin in the extracts was carried out by the chromatographic spectrophotometric method at a wavelength of 282 nm with the calculation of it in the sample through a specific absorption index of 72.23. The quantitative determination of tannins in the extraction was carried out according to the pharmacopoeial method 1 for the determination of tannins in raw materials and medicinal preparations. The dry residue, the pH of the extracts, was determined by pharmacopeia techniques and methods. The results are statistically processed. Results. The studied water extracts of badan made on two compositions and technologies were liquid of a yellow-brown color with a weak smell, astringent taste. The dry residue was 0.022-0.025%, the content of arbutin was 0.34-0.36%, tannins 2.07-2.57%. The pH of the extraction from the powdered raw material in the filter bag was 5.45, from the crushed material 6.58. Conclusion. In the work for the first time, a complex technological and analytical study of aqueous extracts from the rhizomes of badan made according to the technology and the compositions given in the instructions on the packaging with raw materials was conducted. Modern pharmacopoeial methods of qualitative and quantitative analysis identified the active substances phenol glycoside arbutin and tannins. Quality indicators have been established by description, dry residue, pH, arbutin content, tannins.