Optimization of DNA Extraction and PCR Conditions for Genetic Diversity Study in Artemisia Herba- Alba from Algeria
Journal: Scientific Journal of Genetics and Gene Therapy (Vol.2, No. 1)Publication Date: 2016-12-30
Authors : Benchohra Maghni Youcef Bougoutaia Khadidja Abderrabi Ahmed Adda; Othmane Merah;
Page : 010-012
Keywords : Artemisia herba-alba; Genetic diversity; ISSR; Algeria;
Abstract
Background: Artemisia herba alba is an aromatic species very rich in secondary metabolites that can be used in traditional medicine.
The context and purpose of the study: This species suffers from the degradation of its natural habitat. The creation of collections containing diverse ecotypes is important for its preservation. This species contains essential oils which limit the extraction of DNA. The objective of this study is to optimize the DNA extraction and PCR conditions. The method will be used to evaluate the genetic diversity of Artemisia herba alba in the highlands of Western Algeria as a starting point.
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