INSTALLATION OF METROLOGICAL CHARACTERISTICS OF THE BIOTECHNOLOGY METHOD FOR DETERMINATION OF ACUTE LETTAL TOXICITY OF WATER ON ECONOMIC DEVELOPMENT CERIODAPHNIA AFFINIS LILLJEBORG

The use of bioanalysis to assess environmental pollution, in particular for aquatic ecosystems, has become relevant and has become widespread in recent decades. The standardization of the conditions of the bio test should be better defined to ensure applicability as an effective analytical tool. The results of experimental researches on the establishment of metrological characteristics of the biotesting technique for the determination of acute water toxicity using crustacean Ceriodaphnia affinis Lilljeborg are presented. Biotesting was performed using a reference substance of potassium bromochloride (K2 Cr2 O7 ). The method of biotesting for the determination of acute lethal toxicity of water on crustacean Ceriodaphnia affinis Lilljeborg is based on the determination of the difference between the number of dead tseriodafni in tested water and the amount of water in it that does not contain toxic substances – control. The criterion for acute lethal toxicity is the death of 50% ceriodaphnia and more in the experiment compared with the control for 48 hours of bioassay. The following metrological characteristics are established for the method of biotesting to determine the acute lethal toxicity of water on crustacean ceriodophytes: the biotesting result is 0.65 mg/dm3 (61%); Reproducibility of biotesting results is 0.33 mg/dm3 (31%); the standard of operational control of the reproducibility of biotesting results is 0.91 mg/dm3 ; The reaction range of the ceriodaphne is 0.9-3.3 mg/dm3.