Isolation and Diagnosis of Staphylococcus lentus from Different Operation Theater Hospitals

Objectives: Hospital environment play critical role in nosocomial infection, because this environment contains various microorganisms. A frequency of increased pathogenic bacteria in the environment of the hospital is associated with a surroundings rise in different types of nosocomial infections. Methods: A total of 500 samples were collected from environmental samples and clinical samples were distribution on Basra hospitals during the period from October 2016 to February 2017. All isolates were subjected to the cultural, microscopical ,biochemical examination and identification by 16S ribosomal DNA (16S rDNA) and Sequencing of 16S rDNA. Results: It was found that 204 (40.8%) of the total bacterial agents in all surgical theaters with a significant statistical superiority in the prevalence of positive bacteria 187 (91.66%)in different surgical theaters. The study included the diagnosis of 53 isolates (91.37%) Gram positive cocci formulations giving 47 isolates (81.03%) of Staphylococcus species as follows: 17 isolation of Staphylococcus lentus (29.31%). Staphylococcus lentus was Sensitive to Benzyl penicillin, Oxacilin, Gentamicin, Teicoplanin, Tigecycline, Trimethoprin and Nitrofurantion,Levofloxacin, Rifampicin. While S.lentus was Resistant to Erythromycin, Levofloxacin, Vancomycin, but S. lentus was Intermediate to Moxifloxacin Clindamycin, Teteacycline , Fosfomycin. Conclusion: The emergence of a new type of bacteria Staphylococcus lentus which has not been isolated from hospitals previously. Staphylococcus lentus are considered as a pathogenic bacteria and it has the ability to pathology as well as multiple resistance to antibiotics.