MORPHOFUNCTIONAL CHARACTERISTICS OF NEW STRAINS OF DIPLOID EMBRYONIC FIBROBLASTS FOR CELLULAR THERAPY

Among a large number of cell types, which are capable to deliver clinical effect, fibroblasts attract special interest, representing the population of mesenchymal origin cells. Fibroblasts do not only optimize functioning conditions and proliferation of other types of cells, but also play a significant role in regulation of cellular interactions and maintenance of homeostasis in human organism. The ability of fibroblasts to synthesize extracellular matrix proteins and cytokines, to induce migration and proliferation of different cell types in skin damages makes them perspective for widespread clinical application in regenerative medicine. Despite the active development of cellular technologies, at present they are introduced into the wide clinical practice in no one country of the world. Nevertheless, by the experts opinion, in the orld market there may be appear cell resources which ermitted to use and certified reagents that are necessary for its carrying out. The cost of acquisition of such products and technologies will frequently exceed the cost of the similar domestic products which development and implementation now have range of barriers, among them are standard and legal, customs, resource and financial problems. Besides the western countries, the similar developments are conducted in Russia, Republic of Belarus and Ukraine. Considering that each population is genetically unique, the cellular researches of the Kazakhstan population have no alternatives. The goal of this work is receiving new strains of diploid fibroblasts from skin-muscle tissue of the person. Here we define cultural and morphological, cytochemical and karyological characteristics of the isolated allogeneic diploid cultures. We carried out control on biological safety according to WHO requirements. It is revealed that both cultures have properties typical for fibroblasts and do not contain extraneous bacteria, viruses, mycoplasma and fungi. Grown-up in vitro fibroblasts preserve the physiological functions under the corresponding conditions of cultivation and could be used in the form of an aerosol or in the form of cells on a biodegradable scaffolds for treatment of burn atients, patients with injuries of musculo-skeletal system, at recovery and remodeling of functions of the damaged organs and tissues.