IN VITRO EVALUATION OF A DIET SUPPLEMENTED WITH Mn ON NUTRIENT DIGESTIBILITY AND RUMEN FERMENTATION PATTERN IN CATTLE

An experiment was conducted in 3 male rumen fistulated cattle fed on rice straw and concentrate based total diet to study the effects of supplementation of Manganese (Mn) from inorganic source (manganese sulphate) on in vitro dry matter (DM), organic matter (OM) & neutral detergent fibre (NDF) digestibility (%) & in vitro ammonia nitrogen (NH3-N) & total volatile fatty acid (VFA) production. These animals were offered total diet supplemented with 0 ppm Mn (T0) and 24 ppm Mn (T1) as per NRC, 2001 from inorganic source like manganese sulphate. After 21 days of feeding, rumen liquor samples were drawn at different time intervals (24 hour & 48 hour) to study the digestibility of DM, OM & NDF. For estimation of in vitro ammonia nitrogen (NH3-N) & total volatile fatty acid (TVFA) concentration each sample were incubated for 2 hour, 4 hour, 12 hour, 24 hour & 48 hour interval. This study inferred that supplementation of Mn through feed can improve the digestibility and rumen fermentation pattern and there will be more utilization of nutrient (crude protein, ether extract, organic matter). Trace mineral like Mn at per NRC (2001) recommendation was found more effective for the purpose than without supplementation in a diet.