A COMPARATIVE STUDY WITH MURRAH BUFFALO AND INDIGENOUS GIR SPERMATOZOA TO HYPO-OSMOTIC SWELLING TEST
Journal: Exploratory Animal and Medical Research (Vol.3, No. 2)Publication Date: 2013-12-16
Authors : Sumit Chowdhury; Srinibas Das; Tapas Gupta; Debdulal Sana; Subhas Bose;
Page : 159-164
Keywords : ;
Abstract
Hypo-Osmotic Swelling Test (HOST) is a simple but authentic test to measure the integrity of sperm membrane. The present study was undertaken to evaluate the HOST value in Murrah buffalo and indigenous Gir bull in 150 mosm/l tri-sodium citrate and D-fructose HOST solution. Murrah buffalo was having more HOST (49±0.39 %) reacted spermatozoa than indigenous Gir (42±0.57 %) bulls suggesting lesser membrane damage during cryo-preservation and higher fertility rate in Murrah buffalo than Gir cattle.
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