THE STUDY OF ACUTE TOXICITY OF FIRST OBTAINED SALVIA OFFICINALIS EXTRACTS

Antibiotic-associated diarrhea is re- ported in up to 30% of patients treated with antibiotics and up to 12 weeks after completion. It arises from the treatment with antibiotics, without other obvious causes, including acute violation of normal intestinal flora. The most common cause of antibiotic-associated diarrhea among children and adults is Clostridium difficile (C. difficile). C. difficile infection is associated with signifi- cant morbidity and mortality. Drugs of choice for treat- ment of clostridia infections are metronidazole (side effects: loss of appetite, candidiasis, diarrhea, dizziness, headache, nausea, vomiting, ataxia, dark urine, fluffy tongue, hypersensitivity, neutropenia, metallic taste, neuropathy, pancreatitis, seizures, thrombophlebitis, xerostomia, encephalopathy, aseptic meningitis, optical neuropathy, Stevens-Johnson syndrome, toxic epidermal necrolysis, decreased libido); vancomycin (side effects: erythematous rash on the face and upper body, red neck syndrome, hypotension, nausea and vomiting, chills, drug fever, eosinophilia, rash, peripheral edema, urinary tract infection, back pain, headache, reversible neutropenia, phlebitis. Therefore, the actual direction of modern pharmacy is the search and the creation of new medicinal products from plant origin of native origin. Pharmacolog- ical assessment of the safety profile is an important step. Salvia officinalis is a widespread plant on the ter- ritory of Ukraine and is a promising source of raw mate- rials. The interest of both domestic and foreign scientists to this plant increases in connection with the discovery and more detailed study of various classes of biologically active substances and their pharmacological action. The largest number of biologically active com- pounds is contained in the leaf of Salvia officinalis: mon- osaccharides, amino acids, phenol carboxylic and hy- droxycinnamic acids, flavonoids, terpenoids and chloro- phylls a and b, essential oil (1,8-cineol, α-tuyon, β-thyon, camphor, borneol), and others . They have bactericidal, antitoxic, anti-inflammatory, anti-rotten, antispasmodic, secreto-lytic, hemostatic, analgesic, astringent action. According to the literary data, it is known that sage is used in phytotherapy, folk medicine and cooking in many countries of the world. Aim. Study of acute toxicity of the first obtained derivatives of Salvia officinalis extract (extract №1-11). Materials and methods. For single intragastric administration of the studied extracts at doses of 500 mg/kg, 4000 mg/kg, 6000 mg/kg 216 mice (36 groups of 6 animals) were used. The animals were pre-individually labeled and kept on fasting for 3 hours. After passing 14 days, the number of dead mice was determined. They analyzed mortality. Results and discussion. The phytosubstances provided were first obtained and, according to the litera- ture data on medicinal sage, may have a broad pharmaco- logical activity, so the first stage of the study was the study of their acute toxicity. Extracts No. 1-11 are practi- cally nontoxic in class V according to the generally ac- cepted classification by K.K. Sidorov. The results are presented as a table. Conclusions. The acute toxicity of the first ex- tracts of medicinal sage was studied, extracts No. 1-11 belonging to the class V are practically non-toxic accord- ing to the generally accepted classification for K.K. Si- dorov. All the above mentioned can serve as the basis for the further study of Salvia officinalis extracts.