STUDY OF THE MORPHOMETRIC FEATURES OF CAPILLARY NETWORK IN THE EARLY STAGES OF POROUS FIBER MATRIX IMPLANTATIONJournal: Art of Medicine (Vol.3, No. 2)
Publication Date: 2019-05-28
Authors : A.V. Pantus;
Page : 88-92
Keywords : biopolymer; bioimplant; vascularmesh;
The problem facing tissue engineering is to optimize the selection, reproduction and differentiation of cells, to construct matrices or delivery systems, contributing to the maintenance, coordination of tissue regeneration in three dimensions. One of the important criteria that must be taken into account when constructing a matrix is it sability to maintain astablehemo dynamics inside and around the frame work. Currently the interest in biopolymers in bioengineering and medicine is increasing progressively. The materials used in tissue engineering are expected to have some special features and supply engineer and microengineer constructions with characteristics that living tissues have, as follows: the ability of self-repair; the ability of changing anatomy and properties in response to environmental factors. The purpose of the study is to experimentally evaluate the development of the vascular net after two months of subcutaneous implantation of the biopolymer fibrous matrix. The research was carried out on 20 lab animals (rabbits), which were divided into two groups. The first group: surgical intervention was performed to 10 animals, which included the creation of the pocket in the subcutaneous adipose tissue and suturing. The second group: the implantation of the biopolymer matrix into the area between the shoulder blades was performed to 10 animals. The month after the matrix with the underlying tissues was removed. It was divided into 25 parts for histological examination. 9 segments were chosen for analysis: the one from centrally located area, four seg-ments from precentral and four ones from peripheral zones. The histological slides were examined lightopti-cally with the help of the microscope Leica DME with different magnifications. The morphometric features were analyzed using the system for taking microscopic pictures of histological samples (microscope Leica DME and digital camera Nikon P5100) and by using the program Image Tool 2.0 for Windows. The research were conducted at the Department of Pathomorphology and Legal Medicine of Ivano-Frankivsk National Medical University. Statistical analysis was performed with the aid of PC programs Microsoft Exel and Statistica 5.5 (Multi-ple Regression) using the methods of variation statistic and correlation. By using a freezing microtome the slides 30-50 nm were made, then were dehydrated in alcohols of dif-ferent concentrations, soaked in methyl salicylate and fixed in polystyrene. After wards, the slides were exam-ined with the microscope MPS-6. The analysis of the peripheral, paracentral and central zone of the implanted possession of the matrix in the main group is determined by the plane of the cross-section of the vessels from the periphery to the center, which can lead to the active flow of biosynthetic process-es in the central departments of power. material and its preferences from the central zone. This is also evidenced by the presence of venules in the central part of the ma-trix. The location is based on a decrease in the area of connective tissue in 1 section of the court from the pe-riphery to the center. The results display the absence of acute or chronic inflammatory infiltration as well as no transplan-tation rejection. The fiber matrix made by us, creates a peculiar bridge for germination and development of ca-pillary network due to its hygroscopicity and sponginess.
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Last modified: 2019-07-10 17:47:30