AN EVALUATION OF ANTI-UROLITHIATIC ACTIVITY OF POLYHERBAL FORMULATION (LITHOUT TABLET) BY IN-VITRO MODEL

The aim of this study is to prepare & evaluate anti-urolithiatic activity of formulated polyherbal formulation (Lithout tablets) by in-vitro model. The study includes packing of drug and artificial calcium oxalate crystals into the semi permeable egg membrane. After suspending this membrane in the Tris buffer for 7-8 hrs, contents from the membrane collected and added 2 ml of sulphuric acid and titrated with 0.9494 N KMnO4 solutions. 1 ml of 0.9494 N KMnO4 is equivalent to the 0.1898 mg of Calcium oxalate. We have observed that, according to the titration study weight of the calcium oxalate packed in the semi permeable membrane with test drug was less in quantity as that of the initial weight. Thus, it was concluded that the prepared polyherbal formulation (Lithout tablets) significantly produces anti-urolithiatic activity.