Detection of Vibrioes in the Aquatic Environment and the Sewage System by Culture Method in Selected Areas in Yemen| Biomed Grid

Water sources contamination is nearly always the result of human activities and drinking water source is especially vulnerable in areas where population density is high and human use of the land is extensive. When water sources become contaminated, it is difficult to purify. Liquid waste discharged on to the soil initiates solute and microbe movements are the main source that contaminates water sources. Thus far, there are no records of any studies in which Vibrioes contaminations have been studied in aquatic environment in Yemen, so in the present study, we tried to isolate vibrioes in Thiosulfate citrate bile salt media (TCBS) selective media from different sources of drinking water, in addition from swages system in Sana'a city. A hundred and thirty water specimens were collected from drinking water sources that appear to be free from phytoplankton, 70 specimens were collected from drinking water sources that contain phytoplankton from Sana'a city and surrounding villages. 500 ml fresh drinking water or 500ml fresh drinking water contain phytoplankton specimens were passed through the membrane filter, soaked first on alkaline peptone water then 2 loop-full of the culture broth streaked to the TCBS media, and then bacterial growth colonies were identified with standard bacteriological methods. Also 75 specimens from different places of sewage system of Sana'a city were collected. An equal amount of swage's water and enrichment media (alkaline peptone water) were mixed then incubated for 24 hours then sub-cultured to TCBS media after that bacterial growth colonies were identified with standard bacteriological methods. The rate of V. cholerae was zero from drinking water sources or from sewages. V. algenolyticus was isolated from only one fee phytoplankton drinking water sources (rate=0.8%), while in drinking water sources that contain phytoplankton V. algenolyticus and V. fluvialis were isolated with rate equal to 4.3% and 25.7% respectively. The crude prevalence rate of potential pathogenic Enterobacteria was 78.5% for drinking water sources. The rate of Salmonella typhi, and Shigella species, in swages system were 16.4% for both of them, while V. cholerae rate was zero.