BEST GLYCOLYTIC INHIBITOR - D,L GLYCERALHYDE VS SODIUM FLUORIDE FOR ESTIMATION OF GLUCOSE

AIM - To determine an ideal antiglycolytic agent - D,L Glyceraldehyde (GLY) or Sodium Fluoride(NaF) for estimation of glucose. MATERIALS AND METHODS- 80 random blood samples were collected from the OP patient after obtaining explicit consent. 6 ml of venous blood was extracted from each patient and was equally distributed between GLY tube, NaF tube and Plain tube with clot activator(PTC). GLY tube was prepared at the concentration of 5mmolL. Serum from the PTC were separated after 30 minutes and refrigerated at 4C until analysed. Serum from the NaF and GLY coated tubes were put at 25C for a period of 8 hrs. Serum glucose was estimated by glucose oxidase - peroxidase method and the values were compared across the three tubes. STATISTICAL ANALYSIS - ANOVA test was done to compare the values of serum glucose obtained from three tubes. RESULTS - Mean glucose concentration in PTC, NaF tube and the GLY tube were 142.25 mgdl, 124.41mgdl and 139.23mgdl respectively. p-value for GLY tube vs. NaF was 0.483, p-value for GLY tube vs. PTC was 0.970 and p-value for NaF vs. PTC was 0.349 (p value 0.05 considered significant). CONCLUSIONS- For preventing ex vivo glycolysis of blood, DL-Glyceraldehyde coated tubes is as good as PTC tube for glucose estimation by GOD-POD method.