Preparation of permanent neuroanatomical specimens by Klinger's method of dissection and standard S-10 plastination technique

Objective- The aim of this study was to explore the possibility of obtaining lasting neuroanatomical specimens with a combination of Klinger's method and plastination for the purpose of teaching. Methods- Two adult human cadaveric brains, a left cerebral hemisphere and an entire brain, were prepared by the method described by Klinger (1935). Blunt dissection was performed to demonstrate the white matter bundles. Special attention was given to the superior longitudinal fasciculus and the corpus callosum. As the dissection progressed, photographs of each progressive layer were obtained. The dissected specimens were plastinated using standard S-10 plastination technique. Results and Conclusions- Freezing and thawing as used by Klinger's method showed a clear distinction between the gray and white matter and demonstrated the ease of dissection of white matter bundles. Plastination helped to obtain lasting neuroanatomical teaching tools.