Anti-Enzymatic Potential of Two Different Varieties of Malus domestica, (Malus Communis Desf, Ripe and Unripe Fruits) and their Constituent’s Analysis by Gas Chromatography and Mass Spectrometry (GC-MS)

The paper presentscomparative study of urease and alpha-amylase inhibitory activities in two varieties of Malus species, i.e. unripered apple and gold coloured ripe apple. It is very important to know the anti enzymatic potential of these two varieties because the enzyme inhibition can be therapeutic and cure illness caused by malfunctioning of some enzymes. Objectives of this study was to determine the presence of chemical constituents, their role and inhibition activities of urease and alpha-amylase in selected apple varieties. Gas Chromatograph-Mass Spectrometric (GC-MS) was used to identify their chemical constituents. Both varieties exhibited profound activities of urease and alpha-amylase that increased by the increase in the sample's concentration. The IC50 (μg/mL) were 19.77, 19.60, 19.95 and 99.92 for red delicious and golden delicious respectively, whereas, thiourea standard was at 107.90 μg/mL. The IC50 of unripe and ripe fruits were respectively 0.063, 0.051, 0.06 and 0.065 μg/mL comparing with acarbose standard, i.e. 0.044 μg/mL. GC-MS confirmed the presence of 1-ethyl-3-methylbenzene, 1,2,3- trimethylbenzene, 4-methyl-2-furancarboxaldehyde, cymene, glyceric acid, 1-deoxy-2,4, 3,5-dimethylene-d-xylitol, 1,2-butanediol,1-(2-furyl)-2,3-dimethyl, 2,4-dihydroxy-2,5- dimethyl,1-3-furan-3-one 2,4-dihydroxy-2,5-dimethyl-3(2H)-furan-3-one and 2,3- dihydro-3,5-dihydroxy-6-methyl(4H)-pyran-4-one in the selected samples.