CORRECTION OF NEUROTRANSMITTER DISTURBANCES IN HYPOTHALAMUS AND STRIATUM OF RATS IN CHRONIC AND INTERMITTENT ALCOHOL INTOXICATION

Background. Long-term alcohol consumption leads to changes in the brain through the interaction of ethanol with neurotransmitter systems. The intermittent alcohol intoxication is a new experimental state in narcology and its correction is of a great practical importance. Purpose. Assessment of the effect of chronic and intermittent alcohol intoxication on the component content of dopaminergic and serotonergic neurotransmitter systems and pool of neuroactive amino acids in the hypothalamus and striatum of rats, as well as the correction of the revealed disturbances. Material and methods. 76 albino male rats with a weight 180-220 g were used for the experiment. The contents of free amino acids and biogenic amines were determined by HPLC. Correction of neurotransmitter disturbance was performed using «Tritarg» and «Titacin» amino acid composition. Results. In the hypothalamus in chronic and intermittent alcohol intoxication with 4-day interval concentrations of dopamine, tryptophan and 5-hydroxytryptophan increase, and in intermittent alcohol intoxication with 1-day interval the levels of tyrosine, homovanillic acid and noradrenalin increase. In the striatum in chronic alcohol intoxication the levels of tyrosine, dopamine and biochemical parameters of the serotonergic system increase, and with both forms of intermittent alcohol intoxication concentrations of tryptophan and 5-hydroxytryptophan increase. The introduction of the amino acid composition «Tritarg» normalizes the parameters of the serotonergic neurotransmitter system in the hypothalamus, «Titacin» does not have a corrective effect. Conclusions. Chronic and intermittent alcohol intoxication with 4-day interval causes the similar changes in striatum and hypothalamus which is expressed by the activation of dopamine and serotonin metabolism. In intermittent alcohol intoxication with 1-day interval the severity of neurotransmitter disturbances in these parts of the brain was slightly lower.