THE EFFECT OF 5 µM DOSAGE OF AMLODIPINE ON NRF2 EXPRESSION IN NEURON CULTURE INDUCED BY 25 mM OF GLUCOSE

Introduction: Hyperglycemia on neuron cell culture occurs when the cells are induced by 25 mM of glucose. Hyperglycemia state will increase intracellular Ca2+ that leads to increased of oxidative stress in the cell. This causes the activation of antioxidant enzymes by Nrf2 protein. Amlodipine act on nerve cells through bounding with L-Type Calcium Channel (LTCC). 5 µM of amlodipine has neuroprotective effect on cell culture of neuron by inhibiting cell death. Aim: The purpose of this research is to know the effect of treating amlodipine dose 5 μM towards expression of the protein Nrf2 in SH-SY5Y cell culture induced by 25 mM glucose. Methods: In this research on neuron cell culture induced 25 mM glucose within 6 days and treated with amlodipine and without amlodipine 5 µM. Results: Based on research results obtained test results independent t test (p = 0,324) that there is no change of the Nrf2 expression significant between a given neuron cell culture with amlodipine without amlodipine. Spearman correlation test showed (r = 0.290; p = 0.361) that treating by amlodipine 5 µM not significantly increasing Nrf2 expression. Conclusion: Based on this study it can be concluded that treating by amlodipine 5 µM not give effect against Nrf2 expression on neuron cell culture induced 25 mM glucose.