DIAGNOSTIC PARAMETERS ANALYSIS OF THE IMMUNOBLOTING OF DIFFERENT MANUFAC-TURERS FOR DETERMINATION OF SENSITI-ZATION TO PATIENTS AND EPIDERMAL ALLERGENS IN PATIENTS WITH RESPIRATORY ALLERGIC DISEASES

The aim of the study was to compare the diagnostic parameters of different systems of serological examination by immunoblot method for the determination of sensitization to allergens of tick and epidermal groups in patients with respiratory allergic diseases - allergic rhinitis and bronchial asthma. Materials and methods. In the study, 88 patients with allergic rhinitis and / or atopic asthma were examined with three different methods of specific allergic diagnosis (in vivo and in vitro). Inclusion criteria were a diagnosis of allergic rhinitis (both intermittent and persistent) and / or atopic asthma. The prick-test was conducted according to the classic test method according to the normative documents with commercial allergen extracts. Western blots for the determination of IgE levels were performed using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun). Results and discussion. For the qualitative analysis of the diagnostic significance of the methods, ROC analysis was used, which is a plot of the sensitivity of the method to the relative number of false-negative results. According to the results of the curve of detection of specific IgE by AllergyScreen method for determina-tion of sensitization to allergen D. farinae has excellent diagnostic significance (AUC = 0,930), detection of spe-cific IgE by Euroline method for determination of sensiti-zation to allergen D. farinae - good diagnostic signifi-cance (AUC = 0,724) In both cases, the asymptotic sig-nificance is proved by the lack of agreement of the null hypothesis (in which the true area is equal to 0.5) and the reliability of the values of the AUC area. According to the results of the curve of detection of specific IgE by AllergyScreen method for the determi-nation of sensitization to cat allergen has excellent diag-nostic significance (AUC = 1,0), detection of specific IgE by the Euroline method for determination of sensitization to cat allergen - excellent diagnostic significance (AUC = 1,0 ). In both cases, the asymptotic significance is proved by the lack of agreement of the null hypothesis (in which the true area is equal to 0.5) and the reliability of the values of the AUC area. The results of the two systems for the determination of specific IgE for mite and epidermal allergens by the Rida AllergyScreen and Euroline methods do not always agree very well due to the significant systematic divergence of indicators. The results of determining specific IgE for individual allergens are in good agreement with each other; however, for both rabbit and guinea pig epidermal allergens, both methods do not agree with skin test results using the prick-test method, giving false-negative results. Further analysis of the consistency and diagnostic parameters of the methods for other allergen groups is necessary to summarize all the results of the study.