APPLICATION OF CHEMOMETRIC METHODS FOR DETERMINATION OF CLOTRIMAZOLE AND BECLOMETHASONE DIPROPIONATE IN PHARMACEUTICAL DOSAGE FORM

Chemometry is the use of mathematical and statistical methods to improve the understanding of chemical information and to correlate quality parameters or physical properties to analytical instrument data. In the present work, two chemometric methods, named as principal component regression (PCR) and (PLS) based on the use of spectrophotometric data, were developed for simultaneous determination of clotrimazole (CLO) and beclomethasone dipropionate (BE C) in bulk and cream form. The absorbance of zero order UV spectra of CLO and BE C in the range of 80-400 μg/ml and 2-10 μg/ml, respectively were recorded in the wavelength range 230-272 nm at 3 nm wavelength intervals. Twenty-five (25) mixed solutions were prepared for the chemometric calibration as training set and sixteen varied solutions were prepared as a validation set. The suitability of the models was decided based on the RMSECV, RMSEP and PRESS values of calibration and validation data. The % recovery study of both the methods was compared, and it was found near each other. The assay of CLO and BE C for both the methods was found to be in the range of 99.78 to 101.20%. Hence, the proposed methods can be used for simultaneous analysis of the mixture of the drugs, without chemical pre-treatment, with good speed of analysis.