SIMULTANEOUS DETERMINATION OF FROVATRIPTAN, ALMOTRIPTAN AND ZOLMITRIPTAN IN COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
Journal: Indian Drugs (Vol.49, No. 9)Publication Date: 2012-09-28
Authors : Reddy V. S. Murthy T. E. G. K. Usha Rani N. Rao R. S.; Saraswathi K.;
Page : 27-32
Keywords : *E-mail: sunil4249@gmail.com;
Abstract
A simple, precise, accurate, reproducible, robust reverse phase high-performance liquid chromatographic method was developed for the simultaneous estimation of frovatriptan, almotriptan and zolmitriptan in bulk and pharmaceutical dosage forms. The method was validated as per ICH and FDA guidelines. Analysis of the drugs was performed on Phenomenex Chromosil C-18 (250 x 4.6 mm, 5 mc) column, in an isocratic mode employing methanol, acetonitrile and THF in the ratio of 46:50:04 (v/v/v) as mobile phase. UV-visible detector at 269 nm was found to be suitable for detection. Linearity was observed in the range of 40-100 ppm.The % recovery was found to be 99.51, 99.69 and 99.34 for frovatriptan, almotriptan and zolmitriptan respectively. The % RSD values for method precision was found to be 0.86, 0.65 and 1.28 for frovatriptan, almotriptan and zolmitriptan respectively. Limit of quantification (LOQ) and limit of detection (LOD) values were found to be 0.15, 0.08 and 0.09 ppm. and 0.05, 0.02, 0.03 for frovatriptan, almotriptan and zolmitriptan respectively.
Other Latest Articles
- 景观建筑在园林设计中的应用研究
- PHARMACEUTICAL ANALYSIS STUDY OF MAYUR PUCCHA BHASMA
- SYNTHESIS AND CHARACTERIZATION FOR VARIOUS BIOLOGICAL ACTIVITIES OF TRANSITION METAL COMPLEXES OF QUERCETIN
- LIPID METABOLISM BY NATURAL CONSTITUENTS USEFUL IN THE TREATMENT OF OBESITY
- INSULIN SECRETAGOGUE POTENTIAL OF FERONIA ELEPHANTUM FRUIT EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Last modified: 2020-05-28 14:43:25