Genetic Length and Variation in mtDNA NADH Dehydrogenase Subunit I (ND1) of Sumba Ongole (SO) and Peranakan Ongole (PO) Cattle

This study aimed to evaluate the genetic length and mitochondrial DNA variation in the NADH Dehydrogenase Subunit I (ND-1) of Sumba Ongole (SO) and Peranakan Ongole (PO) cattle. A total of 24 blood samples were taken from 12 SO cattle originated from East Nusa Tenggara and 12 PO cattle originated from East Java. Each blood sample (3 ml) was stored in EDTA-containing tube. Amplification of the ND-1 gene was conducted using Polymerase Chain Reaction (PCR) method with the forward primer of 5'AATGGCCGCACGAGGGTTTTA-3' and reverse primer 5'ATGGAGCTCGGTTTGTTTCTGC-3'. The results of amplification were then sequenced in 1st BASE Laboratory, Malaysia. Genetic variation was analyzed using MEGA 6.0 software and genetic length to reconstruct phylogenetic tree was analyzed using the neighborjoining method. The results showed that there was ND-1 gene variation namely 22 mutation spot in SO cattle and 41 mutation spot in PO cattle. According to their genetic length, SO cattle had the genetic length of 0.009, while PO cattle had 0.05. Those two breeds had a close relationship because still within the same group based on the phylogenetic tree. It could be concluded that both of Sumba Ongole and Peranakan Ongole cattle are descendant of Bos indicus.