The Effect of Maltose in Tris-Aminomethane Egg Yolk Base Extender on Semen Quality of Taiwan Native Rooster Following Frozen Storage

The success of freezing chicken semen is desired for preserving Taiwan native chickens. Semen samples from three Taiwan roosters were collected using massage technique twice a week. Two Cryoprotectant Maltose concentrations, 0.6 and 1.2 g/ml, were added in Tris Aminomethane+20 % egg yolk as the basic semen extender. The purpose of this study was to observe the effect of Tris Aminomethane extender supplemented with maltose on post thawing quality (mass spermatozoa motility, spermatozoa motility, spermatozoa abnormality, and viability) following frozen storage on Taiwan Native Rooster spermatozoa. The experiment was conducted in Animal Embriology Engineering Laboratory of The National Pingtung University of Science and Technology, Taiwan. During the period of one month experiment, feed and water were provided ad libitum for the roosters. All semen samples were diluted (semen:diluent=1:10) with warmed diluents at 30ºC in a water bath. The diluted semen samples were gradually cooled to 4ºC for 4h. After cooling, spermatozoa motility was examined. The samples were packed in 0.25 ml straws and plugged with the straw powder. The straws were kept at 4ºC until frozen, which was carried out by placing the samples in liquid nitrogen vapor (-125 to -130ºC) for 20 minutes, and stored in liquid nitrogen (-196ºC) until examination after 3 hours. spermatozoa motility, abnormality and viability showed significant differences (P < 0.05) when preserved with cryoprotectant when compared to the control treatment. It can be concluded that maltose is a potential protector for improving spermatozoa quality following frozen storage.