IN VITRO ANTHELMINTIC ACTIVITY OF AQUEOUS AND ORGANIC EXTRACT OF ROOTS OF PUNICA GRANATUM LINN

Aqueous and organic extracts of Punica granatum were evaluated for their anthelmintic activity using Indian adult earthworm called Pheretima posthuma. Different concentrations of aqueous and organic extract ranging from 10 to 100 mg/ml were made and tested on said earthworm. These extracts were tested for bioassays which includes time for paralysis and time for death of the worms. Aqueous as well as organic extract exhibited strong anthelmintic activity at concentration of 100 mg/ml. Lower concentrations did not produced significant anthelmintic activity. The standard reference drug which is used for comparing anthelmintic activity of these extracts was piperazine citrate at concentration of 10 mg/ml.