Quantitation of human Telomerase Reverse Transcriptase (hTERT) level in normospermic man using ELISA method

Human Telomerase Reverse Transcriptase (hTERT) has a role to maintain the integrity of telomeres at the ends of chromosome which actively proliferated. This enzyme is found in somatic cells and germ cells, one of which is in human sperm cells. The purpose of this study was to measure the quantity of hTERT level in normospermic man. The method used in this research is quantitative descriptive which used ELISA to determine the level of hTERT in human sperm cells. The results showed that the lowest standard concentration of 0pg/mL produced an average absorption value of 0.000. while the lowest standard d with a concentration of 5000pg/mL produced the highest average absorption of 0.088. In addition, the value of hTERT level in human sperm was 2.5pg/mL . Through the determination of hTERT level in sperm, further analysis can be done regarding the quality and infertility in men.