Tissue culture of Phalaenopsis: present status and future prospects

Phalaenopsis one of the popular cut-flower among the orchid species. The improvement/multiplication of this orchid is very difficult through conventional breeding due to delay flowering and uneven flower characteristics. Therefore, tissue culture techniques have been extensively used for improvement of Phalaenopsis by inducing and selecting somaclonal variants. However, it is difficult to get stable regenerations techniques of Phalaenopsis due to production of phenolic compounds, arising somaclonal variation in the culture and less recovery in the field of the regenerated plantlets. Improved and modified tissue culture techniques providing regeneration from various vegetative parts of plant are needed for industrialization and ex situ conservation of this valuable orchid. In this paper we have reviewed various in vitro propagation methods of Phalaenopsis culture which will be helpful for commercialization of this valuable orchid.