TECHNIQUES IN QUANTIFYING HORMONES IN BIOLOGICAL FLUIDS

Diagnosing a disease leads to proper treatment of a patient. Various diseases are occurred due to hormonal dis-balances within the body. Minor shift of the hormonal concentration within the body fluids, account for various physio-metabolic disruptions. This leads to formation of many diseases. So, regular or periodic diagnosis of the hormones within body fluids are very important in a clinical point of view. To quantify hormones within the body fluids there are a no. of ways to perform. The procedures may be of radioactive principles or non-radioactive ones. These may be Enzyme Linked Immunosorbent Assay (ELISA), Radio-immuno-assay (RIA), Spectroscopy etc. Modern techniques like Time resolved fluorescence, chemiluminescence etc. are also there. Besides, previously used bioassays are there for experimental usage within the laboratory. The ultimate goal is to elevate the speed of sample collection, faster diagnosis of the hormones and thus, proper treatment of the patient by the physician.