Proteolyic and Fibrinolitic Activity in the Kidney Tissue at Acute Kidney Damage and Use of Ceruloplasmin

The system of regulating blood coagulation is an important factor, which affects the function of kidney. The disturbances of proteolysis processes, fibrin formation and fibrinolysis lead to a cascade of intravascular coagulation of blood, formation of micro thrombi in renal tissue. The mentioned consequences serve the key link in pathogenesis of thrombo-embolic complications in acute kidney injury of different genesis. The purpose of the research is to study the proteolysis and fibrinolysis in the renal tissue in conditions of acute kidney injury on the background of ceruloplasmin administration. Material and methods. The experimental studies were performed on white nonlinear rats. The acute kidney injury was modeled by intramuscular administration of 50% glycerol solution at a dose of 8 mg/kg. Ceruloplasmin was administered intraperitoneally at a dose of 7 mg/kg/day. State of proteolysis and fibrinolysis in kidney tissue was determined 24 h, 48 h, and 72 h after glycerol administration and induction of acute kidney injury. The proteolytic activity was estimated by the lysis of colorogenic compounds – azoalbumin, azocasein and azocol. The activity of fibrinolysis in kidneys was assessed by the determination of azofibrin lysis with estimation of the total, non-enzymatic, and enzymatic fibrinolytic activity. Results and discussion: The administration of ceruloplasmin to rats with acute kidney injury leads to increase in collagen, low molecular and high molecular weight proteins lysis. Ceruloplasmin also increases the total, non-enzymatic and enzymatic fibrinolytic activity in renal tissue during 24-72 h of acute kidney injury development. Conclusion: It was noted that ceruloplasmin administration in acute kidney injury normalizes proteolytic and fibrinolytic activity in the renal tissue, preventing thrombosis and hemostasis in kidney vessels, which results in the disease course improvement.