Pro-/Antioxidant System Indexes in Rats’ Kidneys in Conditions of Salt Loading Case on the background of Sublimate Nephropathy

Rats poisoning with mercury dichloride solution leads to destruction of the cell membrane and causes activation of free radical oxidation process of macromolecules. It activates antioxidant defense system of the animal organism, which is responsible for decontamination of active form of oxygen. Processes of antioxidant protection play an important role in pathogenesis of different diseases, because emergence of imbalance between activation of macromolecules' free radical oxidation and failure of antioxidant protection system can speed up the development of different pathological processes that are the basis of the renal diseases. Material and methods. The processes of molecules peroxide oxidation in rats kidneys in case of sulema (mercurius corrosivus) nephropathy with salt loading were investigated on white male rats. The content changes of thiobarbiturate acid products and oxi-modified proteins in rats' kidneys in case of salt loading on sulema nephropathy background were found out. It was registered that salt loading of 3% or 0.75% led to content increase of TBA-RP in comparison with control in different kidneys layers. The effect of 0.1 % sulema (mercurius corrosivus) solution in dose of 5 mg/kg of the body weight of animals caused increased indexes of free radical oxidation products, both lipids and proteins, under both types of salt loading relatively to control. For instance, we found that 0.75% salt loading caused increase of TBA-RP content indexes in comparison with the control by 63% in the renal cortex, in 2 times in the renal medulla, and in 2.5 times in the renal papilla in accordance with control. We studied the changes of glutathione-S-transferase and catalase enzymes activity from the antioxidant protection system. We found the decrease of catalase activity in the renal medulla and papilla in case of salt loading after influence of mercury dichloride. However, animals' intoxication with sublimate caused glutathione-S-transferase activity increase in the renal cortex and medulla in comparison with control regardless of salt loading concentration. In conditions of sublimate intoxication and 0.75% salt loading the same index decreased for renal papilla in comparison with control values and did not change at 3% salt loading. Therefore, rats' intoxication with mercury dichloride solution leads to the destruction of the cell membrane and causes activation of macromolecules' free radical oxidation process. In turn, this stimulates the antioxidant system of the animal's organism, which takes part in neutralization of oxygen active forms