Influence of Prolonged Using of Proton Pump Blockers on the Stomach and Colon Microflora in Rats

The purpose of this work was to assess the quantitative and qualitative composition of the microflora in the stomach and large intestine after 28 days of omeprazole and pantoprazole administration. Materials and methods. The studies were conducted on 30 white nonlinear probes-males weighing 160-180 g, which were randomized, divided into three groups of 10 animals in each. Manipulation with animals and their content in the university were carried out in accordance with international recommendations and national legislation on medical and biological research and confirmed the conclusion of the ethical committee of the Taras Shevchenko National University of Kyiv. The first (control) group included rats, which were injected 0.2 ml of water for 28 days intraperitoneally (i.p.). The rats of the second group were injected 14 mg / kg of omeprazole (manufactured by “Sigma-Aldrich”, USA) once daily for 28 days, which was dissolved in 0.2 ml of water for injection. Rats of the third group administered pantoprazole (OM) at a dose of 0.57 mg / kg once per day for 28 days (“Ulsepan” manufactured by “World Medicine”, UK), dissolved in 0.2 ml of water for injection. Results and discussion. The obtained results showed that qualitative and quantitative composition of stomach microflora was changed in both experimental groups after 28 days of suppression of HCl secretion in rats' stomach by omeprazole and pantoprazole. The rate of sowing lactobacillus was significantly decreased. The concentration of Enterobacter increased in the first experimental group, but it was even lower in the second group than in control. The quantitative and qualitative indexes of rats, which were injected by pantoprazole during 28 days, did not differ from the control values of sowing of such representatives of the conditionally pathogenic microflora from the stomach, as Klebsiella, Proteus, Staphylococcus aureus at all, and Staphylococcus epidermidis almost. There was an imbalance between the parameters of the conditionally pathogenic and normal microflora of the rats' intestine of both experimental groups. However, the concentration of such conditionally pathogenic microorganisms as Escherichia coli (hemolytic), Escherichia coli (with altered fermentative properties) in the second group was almost different from the control values. In this case, the indicators of Escherichia coli (lactose negative), Klebsiella, Staphylococcus epidermidis (with hemolysis) and the Candida species fungi were the same as in the control group. Conclusions. Suppression of gastric secretion of hydrochloric acid of the rats by omeprazole during 28 days led to the development of dysbiosis in the stomach and colon. Suppression of gastric secretion of hydrochloric acid of the rats by pantoprazole during 28 days to a lesser extent led to microbiological shifts in the stomach and colon compared to the group, which was administered omeprazole. Prospects for further research will deal with a study of quantitative and qualitative composition of microflora in the stomach and colon under the conditions of modeling of prolonged gastric hypochlorhydria by other blockers proton pumps, and the correction of this condition by various probiotic preparations.