Green Synthesis Of Silver Nanoparticles, Method Development And Validation By UV Spectroscopy In Bulk Pharmaceuticals

The silver nanoparticle is an antibacterial, nontoxic agent. Its conventional synthesis usually generates toxic waste while reaction proceeds hence, green synthesis approach is used have for synthesis of silver nanoparticles (Ag-NPs) by using Datura metel leaf extract as reducing agent. The colour change from colourless to brown was the visual identification for formation of Ag-NPs and showed absorption at 447 nm. The Ag-NPs were of size 180 nm analysed by SEM and characterised by FTIR, where carboxylic peak was obtained at 1569 cm-1, besides confirming presence of alcoholic group and polyphenols. Further, validated method was developed and linearity was found to be 0.995 and % RSD of validation parameters was found to be within the limit <2%. A simple, lab scale, rapid, accurate, precise and sensitive spectroscopic method has been developed and validated as per ICH guideline for synthesized Ag-NPs in bulk pharmaceuticals by UV vis spectrophotoscopy and it is applicable for routine analysis.