Green Synthesis of Silver Nanoparticles and its Antimicrobial Activity

Silver Nanoparticles (Ag NPs) are being used to reduce as antimicrobials. These particles can be incorporated in materials and cloth exposes them sterile. Aqueous silver ions can be reduced by aqueous extract of plant parts to generate particularly stable Ag NPs in water. Apart from being are environmentally friendly process, use of Saraca asoca leaves extract might add synergistic antibacterial effect of Saraca asoca leaves to the biosynthesized nanoparticles. In this paper the biosynthetic production of Ag NPs by aqueous extract of Saraca asoca leaves and its bactericidal effect against E. coli were studied. Ag NPs were synthesized by 3h heating of Saraca asoca leaves extract (10% w/v) and 2 mM AgNO3 solution in 1:3 mixing ratio at room temperature. The synthesized particles were characterized by UV visible spectroscopy, transmission electron microscopy. The effect of temperature, concentration of Saraca asoca extract and AgNO3 solution on the size of silver nanoparticle has been studied. The antibacterial property of the nanoparticles against E. coli was also investigated.