The Cytotoxic Combined Effects of Mixtures of Copper Oxychloride and Chlorfenapyr in HepG2 Cells and Postnatal Model of Toxicity in Female Sprague Dawley rats and its Pups

Previously, we reported that each chlorfenapyr, insecticide, or copper oxychloride, fungicide, had cytotoxic, maternal and developmental toxicity. Here, we want to better understand the combined effect of mixtures of both pesticides in rats' dams and its pups and also in HepG2 cells. The results revealed the cytotoxicity of mixtures of both pesticides in rats' dams and its offspring's through induction of biochemical alteration (elevation of liver enzymes activities, creatinine, and cholesterol) , oxidative stress (increases the level of MDA oxidants and reduction of GSH, GST, sod1, catalase induction) and histopathological changes in different organs likes liver, brain, kidney and spleen. Also, both pesticides induced cell death in HepG2 cells and enhanced oxidative stress evidence by increasing the level of MDA and reduction of the level of SOD1 and GSH in cultivated and treated tissue culture. Taken collectively, the mixtures of chlorfenapyr and copper oxychloride had cytotoxic effects on in-vivo and in-vitro cell lines.