Phytosynthesis, Characterization and Antimicrobial Activity of Iron Oxide Nanoparticle using Henna (Lawsonia Inermis)

Nanotechnology is the most capable technology that can be applied in any field of interest. Technology has created an impressive increase in the production of nanomaterial in many sector of the society. The biosynthesis of nanoparticle is the intersection of nanotechnology and biotechnology that has received increasing attention due to a growing need to develop eco-friendly technologies in material synthesis. Hence an investigation has been carried out to synthesize Iron oxide nanoparticle using Henna leaves (Lawsonia inermis) extract, to study the characterization and antimicrobial activities of it. The results of the green synthesized Iron oxide nanoparticle using Henna leaves extract showed the change of colour from orange to black which may be due to Surface Plasmon Resonance phenomenon. The results of UV analysis of iron oxide nanoparticle revealed the absorbance peak at 425 nm. The results of FTIR analysis revealed the functional groups present in the Iron oxide nanoparticle, displays a number of peaks which reflects the complex nature. The results of XRD analysis showed a high crystallinity of Iron sample level with diffraction angle ranged from 30-80° at 2Ɵ. The shape of synthesized iron oxide nanoparticle was found to be hexagonal and size was 2µm in diameter using SEM. The result of antimicrobial activity (bacteria and fungi) of synthesized Iron oxide nanoparticle indicates a potent antimicrobial activity of nanoparticle.