Efficacy of Adapalene 0.1% and Benzoyl Peroxide 2.5% Gel for the Treatment of Acne Vulgaris Using FTIR-ATR Spectroscopic Technique

Acne Vulgaris is the most common skin disease affecting about 85 % of adolescents. Acne, is driven by androgenic hormones, which typically become active during the teenage years. Excess secretion of hormones, combined with bacteria on the skin, and fatty acids within oil glands, cause acne. The aim of the present study is to evaluate the safety and efficacy of the Adapalene 0.1 %& Benzoyl Peroxide 2.5 % (ADP+BPO) fixed-dose combination gel, in a few human (both male and female) for the treatment of Acne vulgaris. Instead of analyzing blood to diagnose Acne Vulgaris, hair could be used to detect acne vulgaris using FTIR-ATR spectroscopic technique. FTIR spectra of the Acne, hair samples, Pre and Post-treatment along with healthy persons were recorded in the mid-infrared region. The biomarkers for the acne vulgaris samples with specific peaks during the before treatment were analyzed and compared with, hair samples of healthy subjects. The absorption values of some of the specific bands of the biomolecules present in the hair samples viz. , Protein, lipids, and Squalene both the pre- and post-treatment subjects are noted. It was observed that these biomarkers are significantly different between pre- and post-treatment hair samples of acne patients and hence the efficacy of Adapalene 0.1 % and Benzoyl Peroxide 2.5 % is estimated. The results are further validated with statistical analysis by applying the dependent t-test, which indicated that the spectral variations are statistically significant.