A Study of Microbial Isolates of Pyoderma in a Tertiary Care Hospital

Back ground: In India, skin infections constitute a large percentage of skin diseases among which pyodermas take a very prominent place. Pyoderma can either manifest as primary or secondary pyoderma. Most cases of pyoderma do not respond to the antibiotics though they were previously very effective for such cases. This emergence of antibiotic resistance poses a serious threat to public health and also poses a big problem to the clinicians. In order to reduce the problem of antibiotic resistance, it is important to survey and screen all clinical isolates for resistance. Aims and objectives: 1. To isolate and identify the organisms from clinical samples of patients with pyoderma.2. To study the antibiotic susceptibility pattern of isolates. Material and Methods: The pus samples from pyoderma lesions were collected by using sterile swabs. The swabs were inoculated on McConkey Agar and Blood Agar and were incubated over night aerobically at 370 C. If growth was obtained the isolated strain was identified as per standard operative procedures. All the isolated bacterial strains were subjected to antibiotic sensitivity test using Kirby-Bauer disc diffusion method. Results: Growth was obtained in 104 (88.13 %) cases Majority were Bullous impetigo cases (38.13 %). Coagulase positive Staphylococcus was isolated in 42.3 % of total cases followed by Coagulase negative Staphylococcus (24.57 %). Candida was isolated in 3.38 % cases and mixed growth was obtained with Cons and Candida in 3 cases (2.5 %). All the isolated bacterial stains were susceptible to Amoxyclav except E. coli (80 %). Candida isolates were more in diabetic individuals. Conclusions: 1. Impetigo was the most common clinical presentation of pyoderma cases.2. Staphylococci was the predominate organism causing pyoderma.3. Candida was also an etilogical agent of pyoderma.4. All the isolated bacterial strains were resistant to Penicillin and susceptible to amoxicillin and clavulanic acid.