Comparative Analysis on the effect of EMS and MSG on the stomata of Tinospora cordifolia(Willd.)

In India, herbal medicines are one of the oldest Medicines. It heals many diseases as mentioned in the Ayurveda and Homeopathy. The plant Tinospora cordifolia (Willd) is also called marginal shrub due to its property of curing a lot of diseases. The Plant material of T. cordifolia were treated with different concentration of two mutagen i, e EMS and MSG. Stomatal studies were done by usual method of peeling or scratching of epidermis with the help of forceps or sharp razor respectively. Stomatal type were determined based on the classification of stomata on the basis of nature and number of subsidiary cells. The stomata type is anomocytic. Stomatal index, length, breadth and area of stomata of T. cordifolia without treatment is highest at the apex (30.852.569, 11.60.382, 8.20.344, 150.09m^29.37). The stomatal index, Stomatal frequency, length, breadth and area of stomata varied with different regions and concentration of mutagens. It was concluded that effect of mutagens decreases the Stomatal Index, length, width and area of the stomata. Plant treated with MSG performed higher transpiration rate, Stomatal Index, length, width and stomatal area in comparison to EMS treated plant.