In vitro Propagation of Cleome gynandra L. using Nodal Explants

Mass propagation in plants of Cleome gynandra L. were regenerated from nodal explants on MS medium containing various concentration of cytokinins (BAP and KIN) ranging from 5 M to 25 M. When compared to KIN, BAP was found to act in response well in shoot multiplication and number of shoots. Large number of shoots was produced from all concentration of BAP and KIN. Maximum number of shoots and highest frequency of 100 % shoot induction was observed on MS medium containing 10 M KIN and BAP. The elongated shoots were then transferred to MS half strength medium augmented with NAA and IAA in various concentrations for root induction. The roots were initiated and well developed in 2M of both the auxins.