Study the Possible Action of Soft Laser on the Histological Structure of the Plasma Cell in the Lymph Node of Mice
Journal: International Journal of Science and Research (IJSR) (Vol.6, No. 3)Publication Date: 2017-03-05
Authors : Ahmed Anwar Albir;
Page : 257-260
Keywords : Soft laser; Plasma cells of the lymph node; Histological alterations; Mice;
Abstract
Purpose The aim of this presented work is to prove that soft laser may alter the histological structure of the plasma cell of the lymph node of laser groups of mice in order to create an immune response. Materials and Methods Twenty four normal adult male of Swiss albino mice were used in this experimental work. They were aged two months and body weight 38-46gm. These mice were divided into three groups (n=8 each). The first group of mice was used as normal control group. Both second and third groups of mice were considered as laser groups and exposed to laser irradiation for 20 and 25 minutes once daily respectively during the entire periodof exposure to laser irradiation (nine days). Laser of gallium arsenide with a wavelength of 904nm was used in this work. After sacrificing all the mice including control group and laser groups, sections of lymph nodes were prepared by using a routine procedure. Histological evaluation of the overallsections of the lymph nodes was performed by light microscopy, and photographs were made at special magnification. ResultsObvious alterations were observed in the histological structure of the plasma cell of the lymph node of the laser groups such as increased size of the cell and multiplication of the nucleus. These alterations were more obvious in the plasma cell of the lymph node of the third laser group of mice in comparison with the alterations of the plasma cell of the lymph node of the second laser group of mice. Conclusion Soft laser was a useful tool in altering the architecture of the plasma cell of the mice lymph node, and indicated that the time of exposure to laser irradiation was successful in making altered architecture of the plasma cell respectively.
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