Study of in-vitro Micro-propagation of Medicinally Important Plant Andrographis paniculata from its Different Parts

Andrographis paniculata is a plant of high medicinal importance. Explants from leaves, stem internodes and seeds had shown different patterns of callus stimulation under similar conditions of light duration, temperature and humidity with fixed concentrations of IAA and kinetin in MS media. During micro propagation, most efficient callus formation was observed in explants prepared from seeds and internodes. Under influence of IAA and kinetin, most viable callus culture was observed in seeds and internodes of stem.18 of 25 nodal segments have developed callus of 4-6 mm with an efficiency of 72 %.21 of 25 seedlings have developed callus of 2-3mm with an efficiency of 84 %. During callus development, few seedlings also exhibited some shoot development.11 of 25 explants prepared from leaves have developed callus of 3-5mm with an efficiency of 44 %.