Normal Saline Rehydration Technique or Carnoy's Solution - What to Choose for Hemorrhagic Fluid Cytology
Journal: International Journal of Science and Research (IJSR) (Vol.6, No. 5)Publication Date: 2017-05-05
Authors : Mudit Sharma; Rateesh Sareen; Ranu Sharma; Anjali Sharma;
Page : 1416-1419
Keywords : NSRT; Carnoys fixative; Hemorrhagic body fluid;
Abstract
It is not uncommon to receive hemorrhagic fluids for cytological examination. The red blood cells impair diagnostic efficacy of smears. The body fluid can be processed in many ways- NSRT (Normal saline rehydration technique) is one such technique. The present study was done to evaluate quality of smears prepared by NSRT and Carnoy�s fixation. The smears were stained by Hematoxylin& Eosin and Leishman stain. The smears were analyzed for nuclear, cytoplasmic, background and quantity of cells. Scores were given from 0 to2. A total of 30 hemorrhagic fluids were taken. NSSRT showed better cell lysis and better background in comparison to Carnoy�s fixative on Hematoxylin& Eosin stain and also on Leishman stain. Hematoxylin& Eosin was better than Carnoy�s fixative technique. NSRT is better technique as it is simple, cost-effective and faster than Carnoy�s technique for hemorrhagic fluids.
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