Allelic variant frequency of the promotor CRYG gene in patients with initial and immature cataract

Purpose. To investigate the genetical precursors of cataract development the next groups were included: patients sufferingfrom cataract (96) and 96 healthy persons. Method. The determination of gamma-crystallin polymorphism (G-47?A) (rs2289917) was provided using PCR method and further analyses of restriction fragment length polymorphism. Results. These allelic variants have the significant different: G/G ? 35,37 %, G/A ? 53,66 %, A/A ? 10,98 %,, and G/G ? 55,06 %, G/A ? 35,96 %,, A/A ? 8,99 % comparing with the control group (p=0,03, by %2-test). Allelic frequency determination also gives us statistically significant differences between cataract patients and control ? minor allele frequency 0,38 and 0,27 correspondingly, p=0,03. Conclusion. Thus data received show that polymorphism of gamma-crystallin gene is associated with the cataract development in Ukrainian population.