Characterization and Anti-Tubercular Evaluation of Mannich base Substituted Imidazole Derivatives

The present research has focused to characterize and evaluate anti-tubercular activity of Mannich base substituted imidazole derivatives. A new series of substituted imidazole derivatives were synthesized by condensation of O-Phenylenediamine with glacial acetic acid and also with different amino acids (valine, glycine) which on further treated with secondary amines in the presence of formaldehyde. All these compounds were evaluated for in-silico anti-tubercular activity (docking on Cytochrome P450 14 -sterol demethylase) and also screened in-vitro anti-tubercular activity at various concentrations against the Mycobacterium tuberculosis H37RV strain by Proportional Sensitivity test method. All compounds were characterized by FT-IR, 13CNMR, 1HNMR and elemental analysis (CHNO). The titled compound exhibited good binding property with molecular target. The compounds (1a& 2a) showed Sensitivity against H37RV strain.