Isolation of Eriocitrin (eriodictyol 7?O-rutinoside) from Orange (citrus sinuses) Peel by Using Reverse Phase HPLC

Orange (Citrus sinuses) is well known for its nutritional and medicinal properties through the world. Citrus belongs to family Rutaceae. In search for anticancer drugs compelling data from laboratories, epidemiologic investigations, and human clinical trials showed that flavonoids have important effects on cancer chemoprevention and chemotherapy. An objective of the present study is to isolate Eriocitrin a flavanone from Orange peel by using Column Chromatography Technique. Orange peel was shadow dried for 7-10 days and defatted using nHexane, then the residue was extracted using Ethyl Acetate at RT overnight. The extract was air dried and suspended in 1ml of ethyl acetate. A silica gel column of size 300mm X10mm was packed and the 1ml of ethyl acetate extract was loaded onto the column and eluted by using 25 ml ethyl acetate, each 5ml fractions. All these fractions were pooled and checked for Eriocitrin content by HPLC analysis. Results showed that Concentration of Eriocitrin in orange peel powder was found to be 31.57g/ml. Purity of Eriocitrin by HPLC in Ethyl acetate fraction was found 52 %.