Study the Properties of Color Image of Different Chromatography Plates to Estimate the Type, Thickness, and Integrity of Plates

The study concern is an analysis of the properties of color images at different wavelengths belongs to the HPTLC, TLC, and preparative TLC silica gel 60 F254 glass plates. Where the silica plates differ from each other depending on the manufacturing method and the thickness of silica on the plates, where the thin layer chromatography is usually done using a thin, uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. The silica gel (or the alumina) is the stationary phase often contains a substance which fluoresces in UV light. So the aim of the work is to estimate the thickness of silica depending on the properties of the color image of the chromatography plate, in addition to edge detection using Sobel operator on the image plate to ensure that the chromatography plate is clean and free from any damage. The results indicate that use of color properties analysis is simple and efficient method for differentiation among types of plates in addition to estimation of thickness and integrity of plates