Effect of the Aqueous Extract of Seeds of Casimiroa Edulis and Certain Drug Combinations on Cardiac Contractility, Phosphorilasea and Adenyl Cyclase Activation in Isolated Perfused Mammalian Heart

Objective To examine the hypotensive effect of the aqueous extract of the seeds of the plant casimiroa edulis on mammalian heart and vasculatiure. Materials and Methods Studies were done on cardiac contractility, phosphorilase and adenyl cyclase in male Dunkin Hartley guinea-pigs weighing between 300-400 g. on perfused heart by the Langendorff technique. Results Positive inotropic and chronotropic effects were observed mediated by cyclic 3', 5' - AMP, which were not abolished by beta adrenergic-blocking agents. These results further indicate that casimiroa edulis has the capacity to activate adenyl cyclase in particulate fractions of guinea-pig heart. Conclusion These results suggest that there are separate adenyl cyclase systems for casimiroa edulis and nor epinephrine. However, definite proof would require physical separation of the two enzymes. The simultaneous use of H1-and H2-receptor agonists and antagonists thus provides a means for effective and selective inhibition of the cardiovascular actions of casimiroa edulis.