Induction of Different Morphological Types of calli from Leaf and Nodal Explants from Pueraria tuberosa (Roxb)

For the development of an effective tissue culture system in Pueraria tuberosa leaf and node explants were used for the induction of callus. The explants were cultured on MS medium fortified with BAP, 2, 4-D, Kn, IAA and NAA in different combinations. Interestingly different types of calli were formed. Bright green and compact green callus was formed from leaf explants on MS medium fortified with BAP + Kn combination and the growth of the callus was also recorded high. Green friable Embryogenic callus was observed from node explants on MS medium with BAP +IAA. Leaching of phenolic compounds was inhibited by increasing the concentration of sucrose from 25 % - 35 %. Both growth and nature of the callus was good from leaf explant, which is so useful for the regeneration studies and secondary metabolites production in further studies.