Effect of Two Isolates of Trichoderma harzianum on Total Nitrogen, Chlorophyll a&b Contents and Yield of Wheat (Triticum aestivum.L) Class Ebaa-95

This study was conducted to evaluate the effect of using two isolates of biocontrol fungi - Trichoderma harzianum. (T. h. a), and Trichoderma harzianum (T. h. t) with rice straw on improvement of growth (total nitrogen and chlorophyll a, b contents in the leaves) and productivity (plant hight, weight of 1000 grains and yield) of a local wheat - class ( Eba a -95). To attaining these aims, two experements were carryied out, the first was a laboratory experiment that included isolation, purification fungi from wheat field. while the second experiment was conducted in at AL- Mishkhab region wheat field, / AL-Najaf province for growing season 2013. The results appear that Aspergillus nieger has the highest frequency 28.31 % at the pre-planting period, while Trichoderma harizianum reached the highest frequency 43.20 % at the end of growing season. The field treatment ( Soil with rice straw that treated by the spores suspension (104) of bio agent T. h. t. ) gave a significant differences of total nitrogen in wheat leaves that reached 2.652 %, in compare with control 2.450 %, the results, also showed a significant differences in contents of wheat leaves of chlorophyll a, b which amounted 3.320, 1.303 mg/gm in compare with control 2.911, 1.110 mg/gm, respectively. The field treatment ( Soil with arice straw treated by the spores suspension (104) of bio agent T. h. t. ) gave a significant differences in productivity parameters of wheat crop, plant hight reached 110.85 cm in compare with control 107.09 cm, weight of 1000 grains gave 33.719 gm than the control 32.131 gm and finally the wheat significantlly gave yield that reached 6.710 t. h-1 in compare with control 6.140 t. h-1.