Pilot Scale Cultivation of Calocybe Indica by Utilizing Reeds as the Substrate and Nutritional Analysis of its Harvested Fruit Bodies

The present study focused on pilot scale cultivation of Calocybe indica throughout the year (12 months) by utilizing reeds as the substrate. The efficacy of reeds as the alternate substrate and recorded the maximum bioefficiency (121.83 %) at the month of April. Totally 71.23kg of fresh fruit bodies were harvested from 65 kg of dry substrate with bioefficiency of 115.74 %. The nutritional parameters of harvested fruit bodies were analysed viz. , carbohydrate (57.27g/100g), protein (28.73g/100g), fat (0.73g/100g), crude fiber (37.52g/100g), ash (8.80g/100g) and folic acid (57.0g/100g). The mineral contents parameters of harvested fruit bodies were analysed viz. , calcium (135mg/100g), iodine (227g/100g), iron (45.91mg/100g), magnesium (177.3mg/100g), manganese (1.5mg/100g), phosphorus (0.88g/100g), potassium (2.92g/100g) and zinc (13.9mg/100g). Among seven vitamins tested vitamin B3 was recorded maximum (22.94mg/100g). Non detectable level of pesticides such as DDT, p. p. DDT, alpha BHC, beta BHC, gamma BHC and delta BHC. Heavy metal contents were recorded such as arsenic, cadmium, mercury at non detectable level whereas chromium (2.07mg/kg), copper (2.41mg/kg), lead (0.22mg/kg), selenium (4.60mg/kg) recorded. Hence the present shows a positive path that C. indica can be cultivated using alternative substrate and its efficacy was tested at pilot scale. As this mushroom known to several medicinal properties this study will be further focused towards pharmaceutical applications.