A NEW UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ERLOTINIB BY DERIVATIVE SPECTROSCOPY

Erlotinib is as an oral anti cancer agent. It is white to cream color amorphous powder, soluble in water, methanol and partially soluble in acetonitrile, acetone, ethyl acetate and hexane. In this study a simple, accurate and precise UV spectrophotometric method was developed in pure and pharmaceutical formulations. The drug showed maximum absorbance at 246 nm in methanol. The developed method was validated for linearity, precision, LOD and LOQ. The linearity of the drug was found at the concentration range of 2 -10 ?g/ml. The results of analysis have been validated as per International Conference on Harmonization (ICH) guidelines. The percentage recovery for Erlotinib was in the range of 99.93 %. w/w. Hence the proposed method can be easily and conveniently used for the estimation of Erlotinib in bulk and pharmaceutical dosage form.