NaF Patch Formulation and Transport Test to Determine Fluoride Diffusion via Mouse Skin as Membrane (Transdermal in Vitro Test)

Background Transdermal route as an innovation of fluoride delivery for increasing enamel resistance has been developed. Based on transport test of NaF solution, fluoride can be absorbed into the skin. Further research on NaF patch formulation need to be developed. Aims of this research are to formulate NaF patch with good physical properties and find out fluoride weight after using transport test of NaF patch via mouse skin. Methodology The design of the research is quasy experimental with post test only control group design and use Franz Like Diffusion cell used as the instrument. Compartment cell was NaF patch fixed on mouse skin that had been prepared before. The compartment cell was Phosphate Buffer Saline solution 0, 1 M pH 7, 4. Several NaF patch based on NaF concentration was determined based on physical appearance. Sample was taken for time interval of 8, 24 and 48 hours and fluoride content was measured by Potensiometer Spesific Ion Fluoride. Result shows that NaF patch can be formulated with maximum NaF concentration around 1.000 ppm. After transport test of NaF patch 1.000, 750 and 500 ppm and 0 ppm as control, fluoride can penetrate via mouse skin. Fluoride weight was 0, 08-1, 73 g, 0, 72-1, 23 g, 0, 33-0, 94 g and 0, 06-0, 1 g respectifully. Based on repeated measured anova there is an influence of transport time and NaF concetration toward fluoride weight.