Optimizing Mitogenic Potential of HFF Cells by Ethanol Extracts of Leaves of Bersama abyssinica Fresen. (Melianthaceae) and Harrisonia abyssinica Oliv. (Simaroubaceae)

The aim of our study was to optimize the mitogenic potential of HFF cells, in order to help the body defends itself more effectively against pathogens during skin infection and fight against skin aging. Therefore using MTT colorimetric assay, with 70 % ethanol extracts of the leaves of Bersama abyssinica and Harrisonia abyssinica to examine the plants. The results showed that the 70 % ethanolic plant extracts are not cytotoxic but have a mitogenic potential. EE70 % of Bersama abyssinica contain a growth factor to promote cell proliferation and the mitogenic effect of H. abyssinica is due to mitochondrial succinate dehydrogenase. The mitogenic potential of HFF cells has been optimized. This study opens a path into the exploration as to the research on natural FGF growth factors and from Bersama abyssinica.